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Kami Export - Sandra Nichols - Bio-Worksheet-Mutat

This worksheet explains DNA mutations: deletions and insertions cause frameshifts; substitutions can be silent, missense, or nonsense. It shows how mutations change amino acids and includes sickle cell anemia as an example of a missense mutation.

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NAME* "F' 'Mutations WorksheetDeletion. Insertion & Substitution*inThere are several types of mutationy..1- —■DELt1LON(a base is lost/ deleted)INSERTION (an extra base is added/irserted)— Deletion A insertion may cause what s called a FRAMESHIFT mutation, meaning the reading " f r a m e "charges, thus changing the amino acid sequence from this point forwardSUBS 111UTION(one base is substituted for another)— I fa substitution changes the amino acid, its called a MISSENSE mutation— I fa substitution does not change the amino acid, its called a 5ILENT mutation— I fa substitution changes the amino acid to a "stop," it's called a NONSENSE mutation\Complete the boxes below. Classify each as Deletion, Insertion or Substitution AND os eitherframeshift, missense, silent or nonsense (Hint; Deletion A Insertion will always be frameshift).1Original DNA Sequence:T A CmRNA Sequence:A C CU G GT TGA ACG C GCGCA CGVGCA C T . .U G AAmino Add Sequence:MetTrpMnMCjfflSlopMutated DNASequence =1T A CWhafsthemRNAsequence?u ftA TCu Q 5T TG*ACG C GC<5 CA C GUGCACT ...UftA(Circle the change)aminoaddsequence?f.,1ethi Oiline-{Stpp)Willchert likely be effects?■■■■<What type of mutation is this?Subs; till tuitionNcf'isnsf-Mutated DNASequence #2TA CGA C CTTiGGC G AC GA CT ...WhafsthemRNAsequence?A U GC)UG3 A AC C GC UG C UGA(Circle the change)amino add sequence?MelSetC , uProLeuProWillthere likely he effects?YesWhat type of mutation15tiu5?|T seMiori Frame Shifl----------------------------------Mutated DNA Sequence #3T A C A C CT T A G C G A C G1A CT...WhafsthemRNAsequence?AUGU G GAA ®CGCUGCUGA(Circle the change)amino add sequence?TrpA«nStrCy«SlapWillthere likely be effects?What type of mutation is this?Sctshtutisi- i lent V_tat.oi. __________MutatedDNA Sequence £4T A C A C CT T H TG C GA C TAC T...Whafs themRNAsequence?A U GU G GAACCGCU GUGA(Circle the change)amino add sequence?MelTrpAshArgStopStopWillthere likely be effects?yes—hat type of mutation is this?s;.a-<,nuiinii h________________
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Biology

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