Intro to Restriction Enzymes
This flashcard set compares the characteristics of prokaryotic and eukaryotic cells, highlighting their differences and similarities. It covers structural features, types of organisms, and shared components like DNA and ribosomes—ideal for biology students studying cell structure.
What is a restriction enzyme?
restriction enzymes are a class of enzymes that identify, bind to the target sequences of DNA(restriction sites), and cut that specific sequence from the DNA molecule.
Key Terms
What is a restriction enzyme?
restriction enzymes are a class of enzymes that identify, bind to the target sequences of DNA(restriction sites), and cut that specific sequence fr...
What makes restriction enzymes?
bacteria & archaea
what function do restriction enzymes serve for the organism?
The purpose of restriction enzymes is to defend against viruses by cutting off foreign DNA(ie. viruses) from a DNA molecule.
How are restriction enzymes used in cloning projects?
When you need to clone a certain gene, you would use the restriction enzyme to cut, or digest, the targeted sequence of DNA in order to administer ...
What is meant by "sticky-ended" and "blunt-ended" cuts when using restriction enzymes?
When a restriction enzyme cuts a part of the DNA molecule, there are two types of results. For "sticky-ended" cuts, these DNA strands will have sin...
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| Term | Definition |
|---|---|
What is a restriction enzyme? | restriction enzymes are a class of enzymes that identify, bind to the target sequences of DNA(restriction sites), and cut that specific sequence from the DNA molecule. |
What makes restriction enzymes? | bacteria & archaea |
what function do restriction enzymes serve for the organism? | The purpose of restriction enzymes is to defend against viruses by cutting off foreign DNA(ie. viruses) from a DNA molecule. |
How are restriction enzymes used in cloning projects? | When you need to clone a certain gene, you would use the restriction enzyme to cut, or digest, the targeted sequence of DNA in order to administer a new set of DNA sequence to create a Recombinant plasmid. |
What is meant by "sticky-ended" and "blunt-ended" cuts when using restriction enzymes? | When a restriction enzyme cuts a part of the DNA molecule, there are two types of results. For "sticky-ended" cuts, these DNA strands will have single-stranded overhangs that are easier to connect by a DNA ligase. Blunt-ended cuts, on the other hand, produce cuts straight down the middle of a target sequence. These strands can still be connected but won't be as easy to ligate together. |