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Bio Lab 3 Cell Membranes Lab Report

A completed lab report analyzing cell membrane structures and their functions in biological systems.

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    1 BioLab 3 Cell Membranes Lab Report I. Diffusion Define the following terms. Solvent Any substance capable of dissolving or dispersing another substance. Solute Any substance dispersed by water. Solution . Taken together, the solvent and the dissolved solute. Diffusion The movement of dissolved molecules from an area of higher concentration to lower concentration. Concentration gradient Is present when there is a difference in the amount of like molecules in two regions. Dynamic equilibrium When the solvent and solute molecules become equally distributed and the gradient comes to exist. EXERCISE 1 – Factors influencing rate of diffusion • Predict how molecule size and temperature will affect the outcome of this experiment . Molecule size will either increase or decrease diffusion based on the temperature. Record the d ata from the information in the lab. Potassium Permanganate R.T. Methylene Blue R.T. Time (min) Total Diameter (mm) Distance of Diffusion * (mm) Rate (mm/min) * Total Diameter (mm) Distance of Diffusion * (mm) Rate (mm/min) * 0 8mm 0mm 0 mm/min 7mm 0mm 0 mm/min 15 14mm 6mm .43 mm/min 9mm 2mm .13 mm/min 30 18mm 10mm .33 mm/min 10mm 3mm .1 mm/min 45 20mm 12mm .27 mm/min 10mm 3mm .07 mm/min Potassium Permanganate 5 Celsius Methylene Blue 5 Celsius Time (min) Total Diameter Distance of Diffusion * Rate (mm/min) * Total Diameter Distance of Diffusion * Rate (mm/min) *

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    2 © Access Learning Systems (mm) (mm) (mm) (mm) 0 11mm 0mm 0 mm/min 6mm 0mm 0 mm/min 15 12mm 1mm .07 mm/min 8mm 2mm .13 mm/min 30 13mm 2mm .07 mm/min 9mm 3mm .1 mm/min 45 14mm 3mm .07 mm/min 9mm 3mm .07 mm/min * Distance diffused = diameter of each reading minus initial diameter Rate = distance in mm/elapsed time (min) Double click the chart below. A spreadsheet will open. Enter the data from the table above into the spreadsheet in the areas provided. When you are finished, click into another area of the lab report. The spreadsheet will close automatically. Do not close the spreadsheet with the X in the upper right corner. Diffusion of Potassium Permanganate and Methylene Blue 0 0.2 0.4 0.6 0.8 1 Initial Read 15 Minutes 30 Minutes 45 Minutes Time Elapsed Movement Rate (mm/min) Potassium Permanganate (Room Temp) Potassium Permanganate (5°C) Methylene Blue (Room Temp) Methylene Blue (5°C) • What effect did temperature have on the rate of diffusion? It either increased or decreased the rate. • How did molecule size affect the rate of diffusion? At first the molecule was large and then it decreased. • What happens to the rate of diffusion over time? Why? It slows down, due to the temperature. • What can you conclude from this experiment? Temperature has an effect on diffusion. II. Osmosis

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