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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Document preview page 1

BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 1

Document preview content for BIO251 Biology Actual Exam With Answers (204 Solved Questions)

BIO251 Biology Actual Exam With Answers (204 Solved Questions)

BIO251 Biology Actual Exam With Answers ensures a smooth exam experience by offering past test solutions.

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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 1 preview imageNEWBIO251-EXAM 2ACTUAL EXAM WITHCORRECT ANSWERS GRADE A+3 main process of DNA replication--answer--initiation-elongation-terminationCentral Dogma--answer-DNA-> RNA-> ProteinThe process by which DNA is used to encode proteinswhat some of the characteristics of transcription/RNA--answer--Only specific regions are copies-RNA strands are produced, which do not bind with the DNA(exist as single strands)-No proofreadingwhat are the 5 steps in transcription--answer-Step 1: RNApolymerase binds to the promoter (a regulatory region directlyupstream of the gene) and initiates DNA unravelingStep 2 : RNA polymerase facilitates the assembly of freeribonucleotides into chains of RNA, using the DNA as a templateStep 3: The RNA polymerase continues along the gene adding tothe RNA chain, which does not permanently pair with the DNA,but exists as a single strandStep 4: RNA synthesis continues until the RNA polymeraseencounters a specific region of DNA known as a terminator
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 2 preview image
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 3 preview imageStep 5: Once the terminator is reached, the RNA polymerasestalls and a rho termination protein facilitates the RNApolymerase and RNA strand to disassociate from the DNAThe DNA is re-wound and the RNA is free to go on to the nextstepname for groups of3 base pairs of the mRNA--answer-codonshow many codons are non-coding or stop codons?--answer-3are non-coding or stop codonsonly 61 of the 64 encode amino acidswhich molecule can bind both RNA and amino acids--answer-T-RNAgroup of three bases on a tRNA molecule that arecomplementary to an mRNA codon--answer-Anticodont-RNA is considered to be "charged"--answer-when an aminoacid is bound to t-RNAhow many different t-RNA there are--answer-64, one for eachcodonthe study of how the heredity material (DNA and RNA) istransferred.--answer-geneticsall genetic material in a cell--answer-genomewhat is the sub division of genome--answer--chromosome-plasmid
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 4 preview imagewhat is the sub division of chromosomes--answer-genes(coding region)non-codingregionwhat composes the DNA back bone--answer-sugarphosphatewhich are the 4 nucleotides in DNA--answer-AdenineThymineCytosineGuanineUracil (only in RNA)what is the difference between bacterial and Eukaryotachromosomes--answer-Most bacteria have only one circularchromosome, whereas eukaryotes tend to have multiple linearchromosomesWhy is DNA semi-conservative?--answer-When DNA divides,each copy receives one half of the original DNA and one half newDNA.enzyme(s) that uncoiled the DNA--answer-topoisomerase andgyrase, and begins at a region called the origin of replicationenzyme that unwinds and begins separating the DNA strands inboth directions--answer-helicaseenzyme that stabilize thesingle stranded DNA--answer-singlestrand binding proteins
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 5 preview imagewhat is required for the addition of nucleotides in DNAreplication?--answer-a free 3' hydroxyl groupenzyme that adds an RNA primer to the strand being replicated--answer-primaseenzyme that makes sure the proper nucleotide is bound, andconnects it to the growing DNA strand--answer-DNApolymeraseDNA lagging strand--answer--DNA is synthesized in pieces-multiple RNA primers are added-assembled together by the enzymes DNA-polymerase andligasethe structures whereprotein synthesis (translation) occurs--answer-Ribosomeswhich are the 2 microbial ribosome subunits--answer-the 30Sand 50S, which come together to form a functional 70S ribosomewhat does the "S" in ribosomal subunits stands for--answer-Svedbergunits, which measures the rate of sedimentationduring centrifugationwhat are the 3 functional domains of the large microbialribosome subunit--answer-Aminoacyl site (A)site of entry for tRNA'sPeptidyl (P) siteSite of peptide bond formation
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 6 preview imageExit (E) siteSite of exit for uncharged tRNA'sribosome binding siteAlso called the Shine-Dalgarno site--answer-16S RNAsequence is complementary to the conserved sequence in themRNA located just in front the of the gene being translatedaligns theribosome on the mRNA so that translation starts atthe correct location3 steps of translation--answer-Initiation:The ribosome subunits come together around the mRNAElongation:Amino acids are added to the growing polypeptide chainTermination:The ribosome-mRNA complex is disassembled and the protein isreleasedinitiation steps (translation)--answer--binding of the 30S(small) ribosomal subunit to the mRNA (Shine-Dalgarnoalignment)-the first charged tRNA binds to the mRNA (N-formylmethionine)-the 50S (large) ribosomal subunit binds to the 30S subunit (N-formyl methionine/p-site)elongation steps (translation)--answer--the next chargedtRNA binds in the A site of the ribosome
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 7 preview image-the polypeptide chain is moved from the tRNA in the Psite tothe amino acid on the tRNA in the A site-the ribosome moves forward along the mRNA by one codonwhat catalyzes the formation of a peptide bond between theamino acids for elongation in translation--answer-Peptidyltransferasetermination steps (translation)--answer--the ribosomeencounters a stop codon (There is no tRNA corresponding to thestop codon)-the release factor (protein) binds to the A-site causing thepolypeptide chain to be released from the tRNA in the P site-the ribosome destabilizes (subunits dissociate from eachother)genetic makeup of an organismAnything an organism can make or do must be encoded for in it--answer-Genotypeobservable or measurable traits an organism hasFor example, the abilityto ferment lactose--answer-Phenotypechange in the genetic makeup (even single bases) of anorganism--answer-Mutation
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 8 preview imageThere are three basic classifications of mutation--answer--Missense mutation (caused by base substitution)-Nonsense mutation(caused by base substitution)-Frameshift mutation (caused by base addition or deletion)type of mutation that occurs when a change in one nucleotideresults in a change of 1 amino acid in a proteinfunction of protein is variable, depends on location.--answer-missense mutationA mutation that changes an amino acid codon to one of the threestop codons, resulting in a shorter.normally a non-functional protein.--answer-nonsense mutationmutation that shifts the "reading" frame of the genetic messageby inserting or deleting a nucleotidelac operon--answer-the operon that controls the metabolism oflactoseis lac operon on or off when glucose is present?--answer-lacoperon isoffallolactose--answer--formed from lactose.-an inducer for lac operon-binds and causes a structural change to the repressor protein,causing the protein to no longer bind DNA, and transcription canoccur.
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 9 preview imagewhat happens when glucose stores have been depleted--answer-the cell then switches to lactose as its energy sourcewhat happens when glucose levels in the cell are low--answer--cAMP builds up which binds to another protein called catabolicactivator protein (CAP), activating the protein-The activated CAP protein binds to the promoter and increasesRNA polymerase bindingWhen we refer to microbial growth, we usually refer to increasesin _______________, not their ________________--answer-thenumber of organisms, not their sizewhen does bacteria obtain their full size?--answer-shortly afterdivisionwhich are the 2 groups of requirement for bacterial growth?--answer-Physical requirementsChemical requirementswhat are the 3 subdivision of physical requirements for bacterialgrowth--answer-Temperature-can range from near freezingto past boilingpH-range from 2-12 depending on bacteriaOsmotic pressure-range from 1% to 30% NaClwhat are the 3 parts ofthe curve on the temperature growthgraph?--answer-Minimum growth temperatureOptimum growth temperatureMaximum growth temperature
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BIO251 Biology Actual Exam With Answers (204 Solved Questions) - Page 10 preview imagewhich are the 5 classifications of bacteria with respect of theirtemperature tolerance--answer-HyperthermophilesThermophilesMesophilesPsychrotrophsPsychrophileswhat is taken in consideration for the classification of bacteriawith respect of their temperature tolerance--answer-theoptimum growth temperaturepH--answer-refers to the acidity or alkalinity of asolutionwhich bacteria tolerates low pH--answer-acidophileshow much percentage of water bacteria are composed of?--answer-70 to 80 %what happens to bacterial cells when adding large quantities ofsugar or salt (hypertonic environment)--answer-cell lysis orcell plasmolysis can occur, killing or damaging the cellname of the type of bacteria that have adjusted to survive andthrive in high salt concentrations--answer-halophilesObligate halophiles--answer-require high salt concentrations,and can survive 30% solutionsFacultative halophiles--answer-do not require high saltconcentrations, but can survive up to 2% salt
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