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BIO94L-W24Samaniego, SamanthaLab17 - Document preview page 1

BIO94L-W24Samaniego, SamanthaLab17 - Page 1

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BIO94L-W24Samaniego, SamanthaLab17

This experiment tests the effectiveness of handwashing and hand sanitizer by swabbing a subject's palm and culturing bacteria on agar plates to compare bacterial presence after each cleaning method.

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BIO94L-W24Samaniego, SamanthaLab17 - Page 1 preview image94L-W24 Electronic Lab NotebookName: Samantha SamaniegoLab Section: Thursday, at 10:00 a.m.Team Name: Team/ Pod 15Team Members: Jaydon Gian-Wa Vasquez, Jack Dodwell Sergott, Conner Dat Ly,Marlen Rocio Tomas-OchoaTable of ContentsTable of Contents2Notebook components3Formatting Instructions3Instructions3Plagiarism4Detailed Guidelines [This is where you would add experiment Title (e.g. "Plating microbes ')]5Template: Experiment TitleExperiment Title78
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BIO94L-W24Samaniego, SamanthaLab17 - Page 2 preview imageNotebook componentsPre-lab (Must be completed before the start of the lab period}Purpose of experimentMaterials/MethodsStep-by-step procedure/methods taken (sketch or flow chart)Hypothesis and predictionsPost-lab (To be completed during/after experiment is carried out)Data/ResultsAnalysis/lnterpretationFuture stepsA detailed explanation of what to include in each section is included below in the Guidelinessection.Why do we do this? Recording procedures in your notebook in advance (including deviationsfrom published protocols), noting observations and data (including procedural mistakes orlast-minute changes) as the procedure is performed (your memory is NOT reliable!), andanalyzing results immediately following the laboratory experiment is critical to ensuringdocumentation is of the highest quality, consistent, and trust-worthy.Formatting InstructionsTo quickly and easily organize your notebook, please use the Headings formatting options.Headings not only change the font characteristics of your text, they also can be used toorganize a document in the table of contents!InstructionsEach experiment (as designated by a different protocol in the lab manual) should be entitledusing the "Heading 1" style.You can use "Heading 2" style for replicative trials within one experiment, or if there arerelated sub-experiments (e.g. Period 1 and Period 2; different host testing, etc)."Insert" a Page Break at the beginning of each new experiment so that the new entry starts onanew page.PlagiarismM uch of your work wilIb e done on tea ms, and using elect ronic documents. While you areencouragedtodiscuss homework and experiments with your teamandshare raw data, youmust complete all written work individually.Copying anyone else's work is plagiarism and isunacceptable. Please do not share your written work with any other student, as you mayalso be found responsible for plagiarism.This includesthelab notebook, captions on figures,answers to homework questions, lab proposals/reports, and all other work.Theone exceptionis that you and your team may use the same hypotheses and research questions verbatim.Please speak to Dr. Ana Gracia Vedrenneifyouhaveany questions about what constitutesplagiarism or need guidanceonh o w t o avoid it,andreview the UCI Policy on Plagiarism.
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BIO94L-W24Samaniego, SamanthaLab17 - Page 3 preview imagePlating MicrobesDates:1/11/2024Purpose of Experiment:The purpose of this experiment is to identify the total amount of bacteria presenton the palm of one of our teammates after using hand sanitizer, and washingtheir hands. We will be doing this by swabbing the study subject's hand andcovering an agar plate with the contents on the agar plate.Materials Needed:3 Petri dishes3 swabstapemarkerhand sanitizersink to wash hands instudy subject's handMethods:Hypothesis and/or expected results:
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BIO94L-W24Samaniego, SamanthaLab17 - Page 4 preview imageHypothesis: Fewer bacteria growth will be present on the petri dish thatcontained the contents of the "washed hand" swab, compared to that of theunwashed hand and sanitized hand.Data/ResultsAn alysis/ 1interpretation:The Petri dishes that included the contents of the washed and sanitized handdid not grow any bacteria colonies.Since these two Petri dishes did not contain any bacteria, we cannot comparethem to each other. However, we can compare the "unwashed1' Petri dish to theother two dishes, and state that using hand sanitizer or washing your hands ismore effective than not doing anything, since there were no bacteria coloniespresent in these two dishes.With the provided results, we can neither agree nor disagree with the hypothesismade. This is because we expected there to be a much greater amount ofbacteria colonies to grow on the "unwashed hands" petri dish, about half of thatamount of bacteria colonies to grow on the "hand sanitizer Petri dish, and nobacteria growth on the ”washed hands1' petri dish. Our results showed different,
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BIO94L-W24Samaniego, SamanthaLab17 - Page 5 preview imagehowever. We got back only one petri dish with bacteria colonies and twospotless petri dishes with no bacteria growth.We could say that the group's Petri dishes that we received most likely did notrub the cotton swab hard enough on the hand of the study subject for the swabto collect bacteria. This can be said because when we looked at our originalPetri dishes, there was a large amount of bacteria colonies on each of thedishes, of course, with diminishing amounts of bacterial colonies as the intensity'of hand care was considered.Future Directions/Next Steps:Something that could be done in the future with this lab would be trying to seewhether different brands of hand sanitizer work the same, or if certain handsanitizer brands cause more bacteria colonies to grow on the Petri dishes. Thiscould also be tested with different brands of soap, seeing if some soaps causemore bacteria colonies than others.Another thing we could do is compare our results to those of other groups. Wecould see if they used different methods/techniques to acquire a more accuratesample to see more bacteria colonies grow.
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