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BIO94L-W24Samaniego, SamanthaLab17

This experiment tests the effectiveness of handwashing and hand sanitizer by swabbing a subject's palm and culturing bacteria on agar plates to compare bacterial presence after each cleaning method.

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    94L-W24 Electronic Lab Notebook Name: Samantha Samaniego Lab Section: Thursday, at 10:00 a.m. Team Name: Team/ Pod 15 Team Members: Jaydon Gian-Wa Vasquez, Jack Dodwell Sergott, Conner Dat Ly, Marlen Rocio Tomas-Ochoa Table of Contents Table of Contents 2 Notebook components 3 Formatting Instructions 3 Instructions 3 Plagiarism 4 Detailed Guidelines [This is where you would add experiment Title (e.g. "Plating microbes ')] 5 Template: Experiment Title Experiment Title 7 8

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    Notebook components Pre-lab (Must be completed before the start of the lab period} • Purpose of experiment • Materials/Methods • Step-by-step procedure/methods taken (sketch or flow chart) • Hypothesis and predictions Post-lab (To be completed during/after experiment is carried out) • Data/Results • Analysis/lnterpretation • Future steps A detailed explanation of what to include in each section is included below in the Guidelines section. Why do we do this? Recording procedures in your notebook in advance (including deviations from published protocols), noting observations and data (including procedural mistakes or last-minute changes) as the procedure is performed (your memory is NOT reliable!), and analyzing results immediately following the laboratory experiment is critical to ensuring documentation is of the highest quality, consistent, and trust-worthy. Formatting Instructions To quickly and easily organize your notebook, please use the Headings formatting options. Headings not only change the font characteristics of your text, they also can be used to organize a document in the table of contents! Instructions Each experiment (as designated by a different protocol in the lab manual) should be entitled using the "Heading 1" style. You can use "Heading 2" style for replicative trials within one experiment, or if there are related sub-experiments (e.g. Period 1 and Period 2; different host testing, etc). "Insert" a Page Break at the beginning of each new experiment so that the new entry starts on a new page. Plagiarism M uch of yo ur work wil I b e done o n tea ms, a nd u sing elect ronic documen ts. While yo u are encouraged to discuss homework and experiments with your team and share raw data, you must complete all written work individually. Copying anyone else's work is plagiarism and is unacceptable. Please do not share your written work with any other student, as you may also be found responsible for plagiarism. This includes the lab notebook, captions on figures, answers to homework questions, lab proposals/reports, and all other work. The one exception is that you and your team may use the same hypotheses and research questions verbatim. Please speak to Dr. Ana Gracia Vedrenne if you have any questions about what constitutes plagiarism or need guidance on h o w t o avoid it, and review the UCI Policy on Plagiarism.

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    Plating Microbes Dates: • 1/11/2024 Purpose of Experiment: • The purpose of this experiment is to identify the total amount of bacteria present on the palm of one of our teammates after using hand sanitizer, and washing their hands. We will be doing this by swabbing the study subject's hand and covering an agar plate with the contents on the agar plate. Materials Needed: • 3 Petri dishes • 3 swabs • tape • marker • hand sanitizer • sink to wash hands in • study subject's hand Methods: Hypothesis and/or expected results:

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    • Hypothesis: Fewer bacteria growth will be present on the petri dish that contained the contents of the "washed hand" swab, compared to that of the unwashed hand and sanitized hand. Data/Results An alysis/ 1interpretation: • The Petri dishes that included the contents of the washed and sanitized hand did not grow any bacteria colonies. • Since these two Petri dishes did not contain any bacteria, we cannot compare them to each other. However, we can compare the "unwashed 1 ' Petri dish to the other two dishes, and state that using hand sanitizer or washing your hands is more effective than not doing anything, since there were no bacteria colonies present in these two dishes. • With the provided results, we can neither agree nor disagree with the hypothesis made. This is because we expected there to be a much greater amount of bacteria colonies to grow on the "unwashed hands" petri dish, about half of that amount of bacteria colonies to grow on the "hand sanitizer Petri dish, and no bacteria growth on the ”washed hands 1 ' petri dish. Our results showed different,

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    however. We got back only one petri dish with bacteria colonies and two spotless petri dishes with no bacteria growth. • We could say that the group's Petri dishes that we received most likely did not rub the cotton swab hard enough on the hand of the study subject for the swab to collect bacteria. This can be said because when we looked at our original Petri dishes, there was a large amount of bacteria colonies on each of the dishes, of course, with diminishing amounts of bacterial colonies as the intensity' of hand care was considered. Future Directions/Next Steps: • Something that could be done in the future with this lab would be trying to see whether different brands of hand sanitizer work the same, or if certain hand sanitizer brands cause more bacteria colonies to grow on the Petri dishes. This could also be tested with different brands of soap, seeing if some soaps cause more bacteria colonies than others. • Another thing we could do is compare our results to those of other groups. We could see if they used different methods/techniques to acquire a more accurate sample to see more bacteria colonies grow.

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