Cell Structure and Function � Tonicity and pH

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COPY OR SAVE TO A WORD PROCESSING PROGRAMto fill out and hand in to your instructorName:___________________________Date:________________________Cell Structure and Function–Tonicity and pHExercise 1: The Case of the Limp VegetableResults:Table 1: Comparison of States of TonicityTestTubeSolute/SolventAppearance ofPotato StripType of Tonicity Gradient: Is the potatostrip in a Hypertonic, Hypotonic orIsotonicat the beginning of this exercise?1Sugar solutionCrispyHypotonic solution2Salt solutionLimpHyper tonic solutionConclusions:A.How does each potato strip look? Which one is limp and which one is crisp?Answer The potato strip in first test tube appears crispy, while in other appears limp.That is the potato strip present in sugar solution is crispy, while in salt solution is limp.B.How can the difference be explained?Answer The difference is due to concentration of solution,C.What caused the change in appearance of each strip?Answer If solution is less concentrated (sugar solution) than potato, its waterenters in it andprovides potato’s strip a crisp appearance, but if it is more concentrated than potato,then,water from potato comes outside and potato’s strip limps.

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Discussion:A.Did the experiment support or refute the initial hypothesis? Explain why.Answer Yes, as initially, our hypothesis was that, salt solution may provide limp appearance topotato, hence, it has been proven true,B.Explain why vegetables stored in a refrigerator over time become limp.Answer As, with the passage of time, dehydration occurs so, due to removal of water, theyappear limp.C.In this exercise two of the three types of tonicity gradients were observed. Suggest anexperiment to provide an example of the third type of tonicity gradient in action.Answer In third kind of experience, we may take a solution, that is isotonic with water and willnot show any effect on its appearance as no, net movement of water will take place.D.What kinds of cell environmental conditions might affect tonicity in cells?Answer Temperature may enhance the rate of above reactions hence , appearance of cell maychange quite earlier.Exercise 2: TurgorResults:Table 2: Comparison of Turgor Pressure States in Elodea Leaf CellsSolute/SolventScientific Term for Condition:Is the leaf in a Hypertonic, Hypotonic orIsotonic at the beginning of thisexercise?Appearance ofCells at End ofProcuedureWaterIsotonicNo change10% Sodium Chloride,NaCl solutionHyper tonicShrinkageConclusion:A.How can the differences in appearance of the cells in the two solutions be explained?Answer Due to water no impact on leaf’s turgor pressure occurs as there is no difference inConcentration of both and it remains turgid as it was in beginning.

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