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Eukaryotic Cell Cycle and Cancer In Depth Workshee - Document preview page 1

Eukaryotic Cell Cycle and Cancer In Depth Workshee - Page 1

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Eukaryotic Cell Cycle and Cancer In Depth Workshee

In-depth exploration of the cell cycle and protein regulators via Click & Learn. Covers cell division in unicellular vs. multicellular organisms, adult regeneration, and the role of growth factors.

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Eukaryotic Cell Cycle and Cancer In Depth Workshee - Page 1 preview imageBic I n t e r a c t i v eClick & LearnStudent WorksheetDieEukaryotic Cell Cycle and CancerIn DepthTo usethis document. fustreadthe fnshuctvns and FAQs This document is made available by the Howard HughesMedical Institute. Using this document you agreefo trse this document in accordancewith the Terns of UseINTRODUCTIONThis handout complements the Click Si Learn T.ne £u.koryoiic Ce.'i' Cyo'e o r d Concer and is intended as amin-depth examination of the cell cycle and the protein players involved. Fora more general overview, please seethe overview version.PROCEDUREFollow the instructions as you proceed through the Click S. Learn and answer the questions in the spaces below.Click on theBackground" tab onthe rightside.1.Compare and contrast the reasons cell division is important for unicellular |single-celled) and multicellularorganisms.Far single-celled organisms division is important because it is tti eir only mode of reproduction, thus they need it toprevent extinction. For Multicellular organisms, cell division is used as a growth mechanism and to replace old and deadcells, allowing the organism to funotion.2.Provide an example of why ceII division re ma ins im portent to an adult orga nism even afte r it is fu Ilydeveloped.For an adult organism, cell division remains essential because it can allow for replacement of dead cells andcan help heal wound s and mend broken bones. It is a basi c functio n i n huma ns to h ave cel I tu mover so t hatyour organs remain efficient and functional3.Vi'hat is the role of growth factors?Growth factors give signals to cells in ord er to make them divide. W i t h o u t these factors there would be no celldivision4.Cells divide, differentiate, or die. What is differentiation?Differentiation is when a cell stops dividinginorder to specialize in a particular function, also changing theirstructure as a result.5.What is apoptosis? Explain its purpose.Apoptosis is used to eliminate unnecessary, damaged, or unhealthy cells through what is in essence aprogrammed death for the cell.E.Orga nism s rr aintai n the ri ghtnum ber of cel Is by regu latlng the celI cycle. W hat are "cell cycl e regu lato rs?Th ey fu nctio n as molecular signaIs to initiate cel I deat h, pro m pt or cease ceII divisio n, o r di rect cel Is to undergodifferentiationCell Cyde« w w .3i aInte -a c±r.e . cnjUpdsted Fea-LBry 2020P a s e l nT7
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Eukaryotic Cell Cycle and Cancer In Depth Workshee - Page 2 preview imageNiniiBtafrilvfMtlveThe Eukaryotic Cell Cycle and Cancer - In Depthfilch 4 LvamItudHWii WoeItihoi'l7.Watch the videoclip of ceils in the small intestine.Name the g e n e r a l l o c a t i o n along the villus where thef o l l o w i n g processes o c c u r :Cell Division:cryptCell D i f f e r e n t i a t i o n :Along i t s j o u r n e y from the crypt to the top of thevillus (the lumen J.Apoptosis:Tip of the villi8.N a me on e ha rm le ss res j It of too little cel I d ivis ion.One h a r m l e s s result is hair loss or b a l d n e s s9.Name one harmless result of toomuch cell division.On e h a rm less res uIt of toomuch ce 11d ivision is wa rtsClick on the section ofthe circlel a b e l e d "Cell Cycle Phases" in the c e n t e rpurplecircle on the right andusethe " O v e r v i e w " i n f o r m a t i o n in the w i n d o w on the left to a n s w e r theq u e s t i o n s b e l o w .10. List, in order, the four events we collectively call thecell cycle." Next to each event, tv rite thecorrelatingcell cycle phase name.a.G rowth - G1b.DNA replication - Sc_p r e p a r a t i o n to divide - G2dDivision - M11. In g e n e r a l , w h a t is the p u r p o s e of a c h e c k p o i n tinthe cell cycle?A c h e c k p o i n t in the cell cycle acts as a point that regulates p r o g r e s s i o n fromone stage of the cycle to the nextw i t h o u t any errors in the cell orinits DNA12. What is one p o t e n t i a l o u t c o m e when errors occur in this highly r e g u l a t e d call cycle process?cancerClick on "Cell Cycle R e g u l a t o r s and Cancer" in the c e n t e r p u r p l e circle on the r i g h t . Use the i n f o r m a t i o n u n d e r" R e g u l a t o r s O v e r v i e w " in the w i n d o w on the left to a n s w e r the q u e s t i o n s b e l o w .13. What type of p r o t e i n thatregulates thecell cycle is e n c o d e d by proto-oncogenes?Proto-oncogenes e n c o d e p r o t e i n s t h a t s t i m u l a t e / p r o m o t e the progression of a cell t h r o u g h the cell cycle.14 . What type of p r o t e i n t hat regulates t he cel I cycle is encoded by t u mo r su p p resso r ge ne s?Cell Cydewww.Binlnb-railr*e.crg_ xfeted Feaiary 2020Ftagel nf7
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Eukaryotic Cell Cycle and Cancer In Depth Workshee - Page 3 preview imagecell cycle regulators15. The most important cell cycle regulators are theCDK1s16. What is a kinase, and what doesitdo?enzymes that phosphorylate other proteins to activate or inhibit their function17. When are CDKs present inside the cell during the cell cycle?always present in the cell, but only become active whenboundto other prateins called cyclins.IB. When are cyclins present inside the cell during the cell cycle?ate G1 phase19. CDKs form molecular complexes with cyclins. What do activated CDK-cyclin complexes do?They activate CDK- cyclin complexes / stimulatingthecell cycleUsing the cell cycle diagram on the right and both links in the center purple circle, complete the table belowfor each phase. Use bullet points and focus on major events that occur during each phase, checkpoint, andregulatory process. Complete the entire row before moving on to the next phase.PHASEPHASE EVENTSCHECKPOINT EVENTSREGULATORY PROCESSESG105- replicate the DMA- confirm that there areno errors in thereplicated DNA1) Growth factors promoteS-phase cyclin expression. 2)S-phase cyclins accumulateactivating the S-phaseCDK-cyclin complex thatpromotes DNA replicationIf DNA damage isidentified, specific proteinsIe.g. - tumorsuppressor genesataxia telangiectasia mutated(ATM) and Breast Cancer 1
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