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THIS TEMPLATE BIO94L-W24Lastname, FirstnameLabNB (

Electronic Lab Notebook for 94L-W24 by Danielle Zhang. Includes pre-lab and post-lab sections, formatting guidelines, and instructions to ensure accurate, organized, and reliable experiment documentation.

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    94L-W24 Electronic Lab Notebook Name: Danielle Zhang Lab Section: Thursday 6:00 pm Team Name: Team Members: Tianna Yu, Jessica Wang t Deborah Qynhunhu Trinh, Danielle zhang Table of Contents Table of Contents Notebook components Formatting Instructions Instructions Plagiarism Detailed Guidelines [This Is where you would add experiment Title (e.g. "Plating microbes j] Template: Experiment Title Experiment Title

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    Notebook components Pre-lab (Must be completed before the start of the lab period) • Purpose of experiment • MateriaIs/Methods • Step-by-step procedure/m ethocis taken {sketch or flow chart) • Hypothesis and predictions Post-lab (To be completed during/after experiment is carried out) • Data/Results • Analysis/lnterpretation • Future steps A detailed explanation of what to include in each section is included below in the Guidelines section. Why do we do this? Recording procedures in your notebook in advance (including deviations from published protocols), noting observations and data (including procedural mistakes or last- minute changes) as the procedure is performed {your memory is NOT reliable!). and analyzing results immediately following the laboratory experiment is critical to ensuring documentation is of the highest quality, consistent, and trust-worthy. Formatting Instructions To quickly and easily organize your notebook, please use the Headings formatting options. Headings not only change the font characteristics of your text, they also can be used to organize a document in the table of contentsI Instructions Each experiment (as designated by a different protocol in the lab manual) should be entitled using the "Heading 1" style. You can use ''Heading 2" style for replicative trials within one experiment, or if there are related sub-experiments (e.g. Period 1 and Period 2; different host testing, etc). ■ insert' 1 a Page Break at the beginning of each new experiment so that the new entry starts on a new page.

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    Plagiarism Much of your work will be done on teams, and using electronic documents. While you are encouraged to discuss homework and experiments with your team and share raw data, you must complete all written work individually, copying anyone else's work is plagiarism and is unacceptable. Please do not share your written work with any other student, as you may also be found responsible for plagiarism. This includes the lab notebook, captions on figures, answers to homework questions, lab proposals/reports, and all other work. The one exception is that you and your team may use the same hypotheses and research questions verbatim. Please speak to Dr. Ana Gracia Vedrenne if you have any questions about what constitutes plagiarism or need guidance on howto avoid it. and review the UCI Policy on Plagiarism.

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    Detailed Guidelines [This is where you would add experiment Title (e.g. " Plating microbes' 1 )] Dates: • Include all dates that you worked on anything related to this experiment and a very brief mention of what you did on that date. Purpose of Experiment: • This must be done BEFORE coming to lab so that you know what you're doing and why. • Brief (one to three sentences) overview of the experiment. State what you are doing, why, and how it connects to the big-picture goals of your research project' the class. Materials Needed: • Must be done BEFORE coming to lab. • Should be in a bulleted /numbered list format. • Wet lab experiments: List of materials (including concentrations, etc) needed for each experiment. o You can copy these from the protocol, but be sure to read the list carefully ana familiarize yourself with it so that you know what you will neeo in lab. • Dry lab/ Bioinfo rmatic experiments: Briefly list bioinformatics materials (files, software, websites, etc) needed for each experiment. Methods: • Must be done BEFORE coming to lab. • Outline the steps of the experiment here. Please do NOT transcribe the protocol verbatim. Instead, sketch out the steps and describe them using your own words. You may also create a flowchart if you prefer. • Include any changes or modifications that you are making to the protocol if applicable. o Once you do the experiment, if you make a mistake, that's okay - just note the mistake in the methods in another color of font. o For example, if you or your partner adds too much of some reagent, your notebook should indicate what the amount should have been, and what was really aoded, and whether that error may have affected the results. • Wet lab experiments: Don't forget temperatures of incubation, volumes of reagents added, types of media, etc. • Dry lab/ Bioinformatic experiments: Describe the experimental procedure, including tools, programs, and files. • Someone should still be able to look at your methods section and repeat the experiment based on what you have written (yes, even for bioinformatic

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