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Biochemistry: ATP Generation from a Fatty Acid

Biochemistry9 CardsCreated 4 months ago

Lauric acid (12 carbons) undergoes 5 beta-oxidation cycles, producing 6 acetyl CoA units. Each cycle yields 1 NADH and 1 FADH₂, resulting in a total of 5 NADH and 5 FADH₂ for the full breakdown of lauric acid.

How many cycles of beta-oxidation are required to fully break down lauric acid (C12) into acetyl CoA?

Lauric Acid = 12 carbons = 6 Acetyl CoA

= 5 B-ox cycles

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Key Terms

Term
Definition

How many cycles of beta-oxidation are required to fully break down lauric acid (C12) into acetyl CoA?

Lauric Acid = 12 carbons = 6 Acetyl CoA

= 5 B-ox cycles

How many acetyl CoA units are produced from lauric acid via beta-oxidation in total?

Lauric Acid = 12 carbons = 6 Acetyl CoA

How many NADH and FADH2 (electron-rich coenzymes) are produced in each beta-oxidation cycle?

Each beta-oxidation cycle produces 1 NADH (step 1) and 1 FADH2 (step 3)

How many NADH and FADH2 (electron-rich coenzymes) are produced in total from the beta-oxidation cycles needed to convert lauric acid (C12) in many units of acetyl CoA?

5 beta-oxidation cycles are required to completely breakdown lauric acid.

5 NADH (step ...

How many NADH and FADH2 (electron-rich coenzymes) are produced in the CAC from a single acetyl CoA (made in beta-oxidation)?

Each acetyl CoA allows one cycle of the CAC to be completed, which creates 3 NADH and 1 FADH2

How many NADH and FADH2 (electron-rich coenzymes) are produced from six acetyl CoA (produce in beta-oxidation) via the citric acid cycle?

One Acetyle CoA = one cycle producing 3 NADH and 1 FADH2.

6 Acetyl CoA = 18 NADH and 6 ...

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TermDefinition

How many cycles of beta-oxidation are required to fully break down lauric acid (C12) into acetyl CoA?

Lauric Acid = 12 carbons = 6 Acetyl CoA

= 5 B-ox cycles

How many acetyl CoA units are produced from lauric acid via beta-oxidation in total?

Lauric Acid = 12 carbons = 6 Acetyl CoA

How many NADH and FADH2 (electron-rich coenzymes) are produced in each beta-oxidation cycle?

Each beta-oxidation cycle produces 1 NADH (step 1) and 1 FADH2 (step 3)

How many NADH and FADH2 (electron-rich coenzymes) are produced in total from the beta-oxidation cycles needed to convert lauric acid (C12) in many units of acetyl CoA?

5 beta-oxidation cycles are required to completely breakdown lauric acid.

5 NADH (step 1) and 5 FADH2 (step 3) are generated. (one each for each cycle)

How many NADH and FADH2 (electron-rich coenzymes) are produced in the CAC from a single acetyl CoA (made in beta-oxidation)?

Each acetyl CoA allows one cycle of the CAC to be completed, which creates 3 NADH and 1 FADH2

How many NADH and FADH2 (electron-rich coenzymes) are produced from six acetyl CoA (produce in beta-oxidation) via the citric acid cycle?

One Acetyle CoA = one cycle producing 3 NADH and 1 FADH2.

6 Acetyl CoA = 18 NADH and 6 FADH2

How many ATP are produced from the electron-rich coenzymes (via the ETC/OP) in the following process:

i) The beta-oxidation cycles needed to convert lauric acid (C12) into many units of acetyl CoA

5 NADH x 2.5 ATP = 12.5 ATP
5 FADH2 x 1.5 ATP = 7.5 ATP
Total = 20 ATP

How many ATP are produced from the electron-rich coenzymes (via the ETC/OP) in the following process:

ii) The citric acid cycles used to break down multiple acetyl CoA (from beta-oxidation).

18 NADH x 2.5 ATP = 45 ATP
6 FADH2 x 1.5 ATP = 9 ATP
Total = 54 ATP

Two ATP are needed to activate the fatty acid (forms acyl CoA) prior to beta-oxidation. How many ATP are produced in the complete breakdown of Lauric
acid (C12)?

Hint: add up the relevant values calculate in g and h and use the information about the number of ATP used for fatty acid activation

6 acetyl coA = 5 cycles = 50 ATP

5 NADH x 2.5 = 12.5

5 FADH2 x 1.5 = 7.5

= 70

minus 2 = 68