QQuestionMicrobiology
QuestionMicrobiology
What is the difference between using 40X and 100X objectives?
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Step 1In microscopy, the objective is the lens system that you use to magnify the specimen.
The magnification power of an objective is usually indicated on the objective lens itself. Both 40X and 100X objectives provide higher magnification than lower power objectives, but they have some differences:
Step 2**Magnification Power:**
- A 40X objective provides 40 times the magnification of the specimen's original size. - A 100X objective provides 100 times the magnification of the specimen's original size.
Final Answer
- A 100X objective typically requires a thinner specimen and better preparation compared to a 40X objective. This is because the higher magnification can make any imperfections in the specimen preparation more obvious. In summary, a 100X objective provides higher magnification and resolution than a 40X objective, but it also requires better specimen preparation, more light intensity, and has a smaller field of view. Choosing between the two depends on the specific requirements of your observation and the characteristics of your microscope setup.
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