ExamName___________________________________MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers thequestion.1)1)Which of the following techniques can be used to quantify protein-protein interactions?A)The two-hybrid systemB)Chemical cross-linkingC)Surface plasmon resonanceD)Co-immunoprecipitationE)Affinity chromatographySHORT ANSWER. Write the word or phrase that best completes each statement or answers thequestion.2)2)Oxaloacetate is replenished via the ________ in plants and bacteria.3)3)The glyoxylate cycle in plants and bacteria can be used for net carbohydrate synthesisfrom fat because isocitrate lyase yields glyoxylate and ________ without the loss of twocarbons as occurs in the citric acid cycle.TRUE/FALSE. Write 'T' if the statement is true and 'F' if the statement is false.4)4)All of the enzymes of the citric acid cycle are located in the mitochondrion.MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers thequestion.5)5)Which of the following does NOT apply to the reaction catalyzed by the pyruvate dehydrogenasecomplex?A)Each intermediate in the five step reaction is able to diffuse to the next active site in asequential manner.B)The process is highly exergonic and essentially irreversible in vivo.C)The product is acetyl CoenzymeA.D)It takes place in the mitochondrion.E)It is an oxidative decarboxylation.TRUE/FALSE. Write 'T' if the statement is true and 'F' if the statement is false.6)NAD+, coenzyme A, thiamine pyrophosphate, lipoic acid and FAD are all cofactors used in thereaction catalyzed by pyruvate dehydrogenase.6)7)7)The citric acid cycle is classed as a reductive pathway as it produces reduced electron carriers.1Preview Mode
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